Company/ Institute Name : Onan Centre for Molecular Investigations (onanbiotech.com)
Training Offered : Molecular Biology Training
Location : Hyderabad
Website : http://www.onanbiotech.com/
Trainings offered in Molecular Biology :
Module I : Nucleic acids
** Introduction to nucleic acids structure and properties
** DNA isolation from
** Biological fluids
** Animal tissues
** Plants
** Bacteria
** Plasmid isolation
Purification of nucleic acids :
** Ethanol Precipitation
** Salt precipitation
** Gel elution by phenol method
** Gel elution by glass wool method
Characterization of nucleic acids :
** Integrity
** DNA staining
** Quantification
** Estimation by diphenylamine method
** Spectrophotometric method
Kinetics :
** Denaturation kinetics (Tm)
** Renaturation kinetics
** RNA isolation from
** Biological fluids
** Animal tissues
** Quantification of RNA by spectroscopy
Restriction analysis :
** Restriction mapping (RFLP)
** Plasmid foot printing
Module II : Proteins
Extraction and characterization of proteins :
** Cell lysis methods
** Isolation of proteins from culture supernatants
** TCA Precipitation
** Acetone Precipitation
Colorimetric Quantification of proteins :
** Biuret method
** Lowry method
** Bradford
** Absorption at A280 A205
Enzymology :
** Screening for enzyme producing organisms
** Isolation of enzymes from different bacterial cultures
** Purification of enzymes
** Mutation studies
** Enzyme Kinetics
** Effect of temperature
** Effect of pH
** Effect of substrate concentration
** Enzyme assays
Module III : Polymerase Chain Reaction (PCR)
Designing Primers for candidate genes :
** Standardization of primer conditions
** MgCl2concentration
** Annealing temperature
** Annealing time
** DNTPs/MgCl2 ratio
** Elimination of PCR based errors by using different polymerases
Conventional PCR
** Hot start PCR
** Allele Specific PCR
** Multiplex PCR
** Semi quantitative PCR
** Reverse Transcriptase PCR
** Real time PCR (Demo)
Site Directed Mutagenesis :
** Creation of mutants by incorporation of mutant base in primer
** Mega-primer based site directed mutagenesis
** Site directed mutagenesis by whole plasmid amplification
Mutation Detection Methods :
** Restriction fragment length polymorphism
** Hetero-duplex analysis
** Single Strand Conformation Polymorphism
Module IV : Recombinant DNA technology
** Culturing microorganisms
** Selection of medium
** Antibiotic Selection
** Mini-prep preparation
** Creation of sticky ends by Restriction endonuclease digestion
** Creation of Blunt ends by Restriction endonuclease digestion
** Amplification of fragments containing T overhangs
** Ligating into cloning and expression vectors
** Ligation of sticky end fragments
** Ligation of blunt end fragments
** T/A ligation
** Enzyme free cloning
** Preparation of competent host cells
** Preparation of ultra competent cells
** Introducing hybrid DNA in to host cells by transformation
** Screening positive clones
** Plasmid size analysis
** Colony PCR
** Releasing insert with restriction enzymes
** Overexpression of the cloned fragments by induction
** Isolation and purification of overexpressed proteins
Read More : http://www.onanbiotech.com/molecularbiology.html
Contact Us :
Onan Centre for Molecular Investigations,
Door No. 14-224/2, Plot No. 13,
Santhosh Reddy Nagar,
Opp. to Andhra Bank,
Mirjalaguda, Malkajgiri,
Secunderabad- 500 047.